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After constructing the plasmids for the mutant Huntington genes tagged with GFP, we were able to image Huntingtin effects on Wild-Type and Hsp42null S. cerevisiea strains. As the image above shows, mutant Huntington with a polyglutamine tail length of 25 repeats has displays no toxicity in the Wild-type and Hsp42null strains. Diffuse backgrounds in both strains are present with no large Inclusion Bodies (IPs). Mutant Huntingon with a polyglutamine tail length of 72 repeats displays toxicity because of the IPs present in Wild-Type and Hspnull strains. As shown above, IPs are less bright and less concentrated in the Wild-Type strain than in the Hsp42null strain indicating that Hsp42 is needed in reducing aggregation.

Above is an image of large Inclusion bodies (IPs) as well as small aggregates in Wild-Type (Left) and Hsp42null (right) strains. IPs are almost twice as likely to form in the Hsp42null strain. However, the number of small aggregates are unchanged in Wild-Type and Hsp42null strains.

 

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