Characterization of a spliceosome protein orthologous to Homo sapiens in Caenorhabditis elegans – BLOG POST 2

During the past several weeks we have gathered information on the protein of interest by reading multiple peer reviewed research articles on the human protein and its function, the goal with this is to be able to have enough background information on the protein to draw out a more specific hypothesis, as well as specifically tailor the following steps in our research.

We have conducted a few successful RNA interference experiments, and we are in the process of being able to capture images of the live animals in which the protein has been deleted. This will allow us to get a clear visual examination. Thus far, all of the RNA interference experiments have shown a large decrease in progeny, about 92% less than the non-treated C. elegans.

At the moment, the questions we have are regarding the point in development in which the embryos  are failing to develop.

During the background research we also discovered that this protein is responsible for DNA damage response and is a protein that is found in the cell cycle checkpoints, so we would like to be able to pinpoint its role in Caenorhabditis elegans embryo development. 

The ultimate goal is to be able to provide a highly homologous model for research of this protein in C. elegans. We hope that by providing this model, the ability of this protein to interrupt rapidly reproducing cells, such as C. elegans embryos may assist in the research for cancer treatment alternatives.

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