Identifying and Characterizing Novel Genes Involved in Glutathione Sensitivity.

During the past several weeks we have collected peer reviewed articles and have hypothesized that fad genes might be a gene of interest responsible in GSH stress.  These genes are involved in fatty acid synthesis and degradation. It is hypothesized that these genes are also involved in the maintenance of the mycolic layer of the mycobacterium which protects the outer layers of the bacterium. This piece of information might prove useful in understanding the cellular interaction between Mycobacterium tuberculosis (M.tb) and glutathione (GSH).

We have since then infected a few Mycobacterium smegmatis with our phAE175 bacteriophage and we noticed that the titer count was rather low. This could be one of two reasons. The major reason can be due to the tween80 that is used in the media to grow the bacteria during infection, studies have shown that tween reduces the effectiveness of bacteriophages. Another reason is due to the fact that the phAE175 are over two years old and many of them are ineffective.

At this moment we are questioning the role that fad genes, the process of mycolic maintenance and GSH sensitivity all play a role in GSH stress. The ultimate goal of this research in the next coming months is to verify that fad as a novel gene involved in GSH stress and also find and characterize other genes to gain a better understanding of GSH toxicity to M.tb


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