We have successfully inoculuated four different cultures of Pseudoalteromoas species of four different origins. Following days of incubation, the cultures were run in the Polymerase Chain Reaction using a 1500 base pair sequence of ribosomal DNA whereas to seek out exclusive protein synthesizing properties. Standard operating procedures, utilizing the functionality of Thermus Aquaticus Polymerse, were performed under the appropriate pressure, temperature, time, and relative controls.
To confirm the preceding amplification, we ran the four PCR products through a stained gel electrophoresis procedure. The model ladder was injected in the right-most lane while the experimentals were injected into the adjacent ones. The equivalence of the base pair sizes (via the migration in the electrolyzed gel over time) is observed to be equivalent. Furthermore, they are all seen to be equivalent to that of the ~1500 base pair size.
We plan to send out the 8F-1508R PCR products for DNA sequencing where we can later assess the base pair sequence in reference to known species of Pseudoalteromoas. Further down the line, we plan to address the antifouling properties of the microbe(s) with experimental surfaces containing synthesized hybrid organic-inorganic polymers.