The 16srRNA gene from cultured isolates from the squid accessory nidamental gland was amplified using PCR and sent out for sequencing. Results from the 8 initial isolates came back, allowing us to identify the different genera of bacteria associated with the squid. The amplified 16srRNA gene that was amplified was almost the entire length of the gene allowing for a reliable identification of each bacterial isolate. The two different primers (8F, 1514R) utilized caused replication from different sides of the DNA strand. From the two independent sequencing reactions, a contiguous DNA sequence was aligned with CodonCode Aligner. We performed a Standard Nucleotide BLAST (NCBI) in attempt to find closely related bacteria and other invertebrates that might be associated.
These basic molecular and microbiological techniques were applied to an unsuspected discovery of invasive Clinging Jellyfish, Gonionemus vertens, that were discovered in July during research in the Long Island Sound. I have isolated 31 different bacterial colonies derived from a single cling jellyfish specimen. We have gone through the process of isolating several samples, amplifying the 16srRNA gene as well as characterizing the basic bacterial morphology. The process of isolating these Clinging Jellyfish bacterial isolates revealed a number of colonies that had a green or orange iridescence. We are interested in identifying these novel bacteria and would like to discover the role of this iridescent bacteria with this jellyfish. After the 16srRNA gene is amplified, cleaned up and sequenced, they will be sent out for sequencing. From there, we will once again be able to further classify and explore the different type of bacteria that are associated with the Clinging jellyfish.