This summer I worked with Professor Mojica and researched the binding capacity of nanoceramics and sulfa antibiotics. This is a continuation of research we completed during the school year. For the summer, we chose to research sulfadiazine and sulfamethoxazole. We tested these antibiotics alongside four types of nano ceramics, titanium oxide, silicon oxide, magnesium oxides and zinc oxide. The purpose of the study was the observe if the absorbance of the antibiotics was altered after being exposed to each type of nanoceramic, which would allow us to conclude that binding had taken place.
We did observe changes in peak absorption after adding nanoceramics. The blue shift of maximum peak absorbance is shown for sulfamethizole with silicon oxide in Figure 1. Reduction in absorbance was observed when sulfamethizole was mixed with aluminum oxide and titanium oxide. The same trend of maximum shift can be seen with sulfadiazine and silicon oxide in figure 2. Obvious changes in the absorbance profiles were shown when Zinc oxide and silicon oxide were added to sulfadiazine. Figure 3 shows a chromatogram of the sulfamethizole solution before and after addition of a nanoceramic (zinc oxide), which shows reduction in the peak area.
A shift was observed when both antibiotics were in presence of every nanoceramic, which allows us to conclude that both sulfadiazine and sulfamethizole bind with aluminum oxide, silicon oxide, titanium oxide, and zinc oxide. The type of interaction depends on each molecule and we have not yet studied how these may affect the structure of the antibiotic. It is possible that new products form when sulfa drugs react with nanoceramics. We hope to continue this study into the next school year in hopes of finding out more about these interactions.